期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 11, 页码 2348-2351出版社
OXFORD UNIV PRESS
DOI: 10.1271/bbb.100465
关键词
plasmid; binary vector; transgenic plant; rice seed; molecular farming
类别
资金
- Ministry of Agriculture, Forestry and Fisheries of Japan [MP2108]
A new set of plasmids for plant transgenic studies was developed. Its strong point is that independent gene cassettes are connected within one binary vector by the restriction endonuclease-based technique only. Using the set, two overexpressing cassettes and three RNA interference (RNAi) cassettes were successfully introduced into rice. Our plasmid set is useful for producing commercial transgenic plants, especially in the case of rice.
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