4.4 Article

A Real-Time SUMO-Binding Assay for the Analysis of the SUMO-SIM Protein Interaction Network

期刊

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 6, 页码 1302-1305

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TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100082

关键词

post-translational modification; SUMO; protein interaction; histone methyltransferase; chromatin

资金

  1. Naito Foundation
  2. Japanese Ministry of Education, Culture, Sports, Science, and Technology

向作者/读者索取更多资源

SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.

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