期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 73, 期 6, 页码 1432-1434出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80888
关键词
Coprinopsis cinerea; glycoside hydrolase family 6; basidiomycete; cellulose degradation; cellobiohydrolase
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Ministry of Agriculture, Forestry, and Fisheries of Japan
Recombinants, Cel6 from Coprinopsis cinerea (CcCel6)A, -B, and -C, were expressed by Escherichia coli. These enzymes produced cellobiose from phosphoric acid-swollen cellulose. When Avicel was used as the substrate, CcCel6A showed higher activity toward the substrate than CcCel6B or -C. In reaction with carboxymethylcellulose, CcCel6B and -C hydrolyzed the substrate, whereas CcCel6A failed to react.
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