期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 73, 期 5, 页码 1209-1211出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80872
关键词
azo dyes; azoreductase; substrate specificity; flavoprotein; Bacillus sp B29
类别
资金
- Japan Society for the Promotion of Science (JSPS)
- Vietnamese Academy of Science and Technology
We cloned and expressed two genes encoding azoreductase homologes, AzrB and AzrC, from Bacillus sp. B29. Purified recombinant AzrB and AzrC were homodimers with 23kDa identical subunits, and were flavoproteins. NADH was preferred as electron donors for both azoreductases. The azoreductases showed optimal activities at 70 degrees C (AzrB) and 55 degrees C (AzrC), and retained activities up to 55 degrees C (AzrB) and 50 degrees C (AzrC) after incubation for 1 h. Other enzymatic properties, including the substrate specificities of both azoreductases, were also investigated.
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