期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 73, 期 12, 页码 2783-2786出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.90584
关键词
Red/ET recombination; glutaredoxin5 (Glrx5); mitochondria; short homology arms
类别
资金
- Hokuriku Innovation Cluster for Health Science
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
The original protocol of Red/ET recombination requires 50-bp sequence homology with target vector on both sides of the DNA fragment. To make it more cost effective, we investigated to determine the minimal length of homology required for the system to work. We found that a homology of 9-bp was sufficient to achieve homologous recombination with more than 50% efficiency.
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