期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 73, 期 11, 页码 2452-2459出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.90407
关键词
Arabidopsis; arabinogalactan protein; early nodulin-like protein; glycosylphosphatidylinositol-anchored protein; phytocyanin
类别
资金
- program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [17380071]
- Japan Society of the Promotion of Science Fellows [12046]
- Grants-in-Aid for Scientific Research [17380071] Funding Source: KAKEN
Early nodulin-like proteins (ENODLs) are chimeric arabinogalactan proteins (AGPs) related to the phytocyanin family. Although they show similarities with other phytocyanins, they lack amino acid residues for copper binding. Despite the existence of other phytocyanins, information about the function of ENODLs in plants is largely lacking. In this study, we characterized ENODL genes consisting of 22 members in Arabidopsis thaliana. Structure prediction indicated that most ENODLs are glycosylphosphatidylinositol-anchored chimeric AGPs. Expression analysis by real-time reverse transcription polymerase chain reaction indicated that most ENODL genes showed spatially specific expression, mainly in the flower organs. Furthermore, we obtained and analyzed 26 homozygous T-DNA insertion lines of 15 ENODL genes, but novel biological roles were not uncovered, probably due to functional redundancy. The detailed phylogenetic and expression analyses and characterization of the available insertion lines in this study might facilitate future studies to elucidate the biological roles of ENODLs.
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