期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 73, 期 1, 页码 67-73出版社
OXFORD UNIV PRESS
DOI: 10.1271/bbb.80477
关键词
Coprinopsis cinerea; glycoside hydrolase family 6; cellulose degradation; basidiomycete
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Ministry of Agriculture, Forestry, and Fisheries of Japan
We searched the genome database of the basidiomycete Coprinopsis cinerea (Coprinus cinerea) and found five genes encoding the glycoside hydrolase family 6 (GH6) enzyme, CcCel6A, CcCel6B, CcCel6C, CcCel6D, and CcCel6E, designated in order of increasing locus number (CC1G_01107.1, CC1G_04166.1, CC1G_08276.1, CC1G_08277.1, and CC1G_10605.1). The amino acid sequence of CeCel6A suggests a two-domain structure consisting of an N-terminal family I carbohydrate-binding module (CBM1) and a GH6 catalytic domain, while the other genes lack CBM1. The transcripts of CcCel6A were observed at the active growth stage in cellulose culture, whereas they were absent from glucose culture. Cellobiose strongly induced transcription of CcCel6A. On the other hand, transcripts of CcCe16B, -D, and -E were detected in both glucose and cellulose cultures, and transcription of them was induced weakly by cellobiose. The transcript level of CcCel6C was not influenced by glucose or cellobiose.
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