期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 73, 期 3, 页码 479-488出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70712
关键词
identification of bacteria; NO3- reducing bacteria; microchip electrophoresis systems; restriction fragment length polymorphism; multiple restriction enzymes
类别
资金
- Cooperative Research Project on Biological Resource Management
- Organization for Economic Co-operation and Development
- Japanese Ministry of Agriculture, Forestry and Fisheries
- Development of New Agricultural Techniques Compatible with the Conservation of Eco-Systems by Innovative Method for the Recycling of Agricultural Resources
Phylogenetic estimation method without determination of DNA sequence was developed. By this method, fragment length polymorphism separately digested with multiple restriction enzymes was measured using microchip electrophoresis and affiliated with those calculated from corresponding DNA sequence in the theoretical database. The phylogenies of 129 NO3- reducing bacteria newly isolated from field soils were estimated by this method, and were compared to those by carbon source utilization profiles and by comparative sequence analysis of 16S rDNA. Various bacteria such as Micrococcus sp. (one isolate), Acidovorax delofieldii (one isolate), Cupriavidus necator (one isolate), Burkholderia sp., (seven isolates), Commamonas acidovorans (two isolates), Herbaspirillum seropedicae (two isolates), Ralstonia sp. (six isolates), Pseudomonas spp. (14 isolates), and Acinetobacter spp. (one isolate), were affiliated as similar to those by sequence analysis of 16S rDNA, while exact affiliation of genus was difficult for those belonging to Enterobacteriaceae.
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