期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 72, 期 10, 页码 2739-2749出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80383
关键词
chemical array; carbonic anhydrase II; small molecule inhibitor; protein library
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
The identification of specific interactions between small molecules and human proteins of interest is a fundamental step in chemical biology and drug development. Here we describe an efficient method to obtain novel binding ligands of human proteins by a chemical array approach. Our method includes large-scale ligand screening with two libraries, proteins and chemicals, the use of cell lysates that express proteins of interest fused with red fluorescent protein, and high-throughput screening by merged display analysis, which removes false positive signals from array experiments. Using our systematic platform, we detected novel inhibitors of carbonic anhydrase II. It is suggested that our systematic platform is a rapid and robust approach to screen novel ligands for human proteins of interest.
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