期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 72, 期 11, 页码 3030-3033出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80410
关键词
acetolactate synthase; asymmetric synthesis; chiral amines; omega-transaminase
类别
资金
- Korea Science and Engineering Foundation
- Ministry of Education, Science and Technology, Republic of Korea
- National Research Foundation of Korea [11-2008-18-003-00] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
To produce (S)-alpha-methylbenzylamine (MBA) from acetophenone, recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase was used as a whole-cell biocatalyst. The solvent-bridge reaction system increased the yield of the whole-cell reaction by 2.5-fold, and the inhibitory (S)-alpha-MBA produced in the omega-transaminase reaction solution (pH 8.0) moved into the extraction solution (pH 3.0) via an organic solvent.
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