期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 72, 期 11, 页码 3021-3024出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80342
关键词
Monascus pilosus; restriction-enzyme-mediated integration (REMI); aurintricarboxylic acid (ATA)
类别
资金
- National Science Council, Republic of China [NSC-95-2218-E-009-018]
- Aiming for the Top University and Elite Research Center Development plan
The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.
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