期刊
BIOSENSORS-BASEL
卷 8, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/bios8010019
关键词
digoxin detection; homogeneous assay; DNA nanotechnology; DNA strand displacement competition reaction; detection of small molecules; detection in plasma
资金
- Innovation Fund Denmark
- Danish National Research Foundation [DNRF81]
The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of the concentration of digoxin spiked into in a plasma sample. The assay is based on a shift in the equilibrium of a DNA strand displacement competition reaction, and can be performed in 30 min for concentrations above 10 nM. The equilibrium shift occurs upon binding of anti-digoxigenin antibody. As a model, the assay provides a potential alternative to current small-molecule detection methods used for therapeutic drug monitoring.
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