期刊
APPLIED MATERIALS TODAY
卷 10, 期 -, 页码 194-202出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apmt.2017.12.004
关键词
3D electrospun nanofibrous scaffold; Hydroxyapatite functionalization; Phenamil; Osteogenic differentiation; Bone regenerationa
资金
- EPSCoR program of National Science Foundation [IIA-1335423]
- Competitive Research Grant program of South Dakota Board of Regents [UP1500172, UP1600205]
- COBRE grants of National Institutes of Health [P20 GM103620, P20 GM103548]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM103620, P20GM103548] Funding Source: NIH RePORTER
- Office Of The Director [1355423] Funding Source: National Science Foundation
Bone morphogenic protein 2 (BMP2) is a key growth factor for bone regeneration, possessing FDA approvafor orthopedic applications. BMP2 is often required in supratherapeutic doses clinically, yielding adverse side effects and substantial treatment costs. Considering the crucial role of materials for BMPs delivery and cell osteogenic differentiation, we devote to engineering an innovative bone-matrix mimicking niche to improve low dose of BMP2-induced bone formation. Our previous work describes a novel technique, named thermally induced nanofiber self-agglomeration (TISA), for generating 3D electrospun nanofibrous (NF) polycaprolactone (PCL) scaffolds. TISA process could readily blend PCL with PLA, leading to increased osteogenic capabilities in vitro, however, these bio-inert synthetic polymers produced limited BMP2-induced bone formation in vivo. We therefore hypothesize that functionalization of NF 3DPCL scaffolds with bone-like hydroxyapatite ( HA) and BMP2 signaling activator phenamil will provide a favorable osteogenic niche for bone formation at low doses of BMP2. Compared to PCL-3D scaffolds, PCL/HA-3D scaffolds demonstrated synergistically enhanced osteogenic differentiation capabilities of C2C12 cells with phenamil. Importantly, in vivo studies showed that this synergism was able to generate significantly increased new bone in an ectopic mouse model, suggesting that PCL/HA-3D scaffolds act as a favorable synthetic extracellular matrix for bone regeneration. (c) 2017 Elsevier Ltd. All rights reserved.
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