期刊
BIORESOURCE TECHNOLOGY
卷 269, 期 -, 页码 32-39出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.08.066
关键词
Docosahexaenoic acid; Schizochytrium sp.; Continuous fermentation; Kinetic modeling
资金
- National Natural Science Foundation of China [21376002, 21476111]
- Jiangsu Provincial Natural Science Foundation [BK20131405]
- National High-Tech R&D Program of China [2014AA021701]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Docosahexaenoic acid (DHA) has wide-ranging benefits for normal development of the visual and nervous systems in infants. A sustainable source of DHA production through fermentation using Schizochytrium sp. has been developed. In this paper, we present the discovery of growth-uncoupled DHA production by Schizochytrium sp. and the development of corresponding kinetic models of fed-batch fermentations, which can be used to describe and predict the cell growth and substrate utilization as well as lipid and DHA production. Based on this kinetic model, a predictive model of multi-stage continuous fermentation process was established and used to analyze, optimize and design the process parameters. Optimal predicted processes of two-stage and three-stage continuous fermentation were developed and verified in lab-scale bioreactor based on the predicted process parameters. A successful three-stage continuous fermentation was achieved, which increased the lipid, DHA content and DHA productivity by 47.6, 64.3 and 97.1%, respectively, compared with two-stage continuous fermentation.
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