期刊
BIORESOURCE TECHNOLOGY
卷 269, 期 -, 页码 195-202出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.08.069
关键词
Sugar beet pectin; L-Arabinose; Arabinofuranosidase; Biomass pretreatment; Continuous bioprocessing
资金
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/K014897/1]
Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel alpha-L-arabinofuranosidase (AF), yielding monomeric L-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据