期刊
BIORESOURCE TECHNOLOGY
卷 271, 期 -, 页码 306-315出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.09.122
关键词
Biomass; Biopolymer; Furfural; Hydroxymethylfurfural; Polyhydroxyalkanoate
资金
- KU Research Professor Program of Konkuk University, Seoul, South Korea
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2017M3A9E4077234]
- National Research Foundation of Korea (NRF) [NRF-2015M1A5A1037196, NRF2016R1D1A1B03932301, 2017R1D1A1B03030766]
- Next-Generation BioGreen21 Program (SSAC), Rural Development Administration [PJ01312801]
- Polar Academic Program (PAP) [PE18900]
Pretreatment of lignocellulosic biomass results in the formation of byproducts (furfural, hydroxymethylfurfural [HMF], vanillin, acetate etc.), which affect microbial growth and productivity. Furfural (0.02%), HMF (0.04%), and acetate (0.6%) showed positive effects on Ralstonia eutropha 5119 growth and polyhydroxyalkanoate (PHA) production, while vanillin exhibited negative effects. Response optimization and interaction studies between the variables glucose, ammonium chloride, furfural, HMF, and acetate using the response surface methodology resulted in maximum PHA production (2.1 g/L) at optimal variable values of 15.3 g/L, 0.43 g/L, 0.04 g/L, 0.05 g/L, and 2.34 g/L, respectively. Different lignocellulosic biomass hydrolysates (LBHs), including barley biomass hydrolysate (BBH), Miscanthus biomass hydrolysate (MBH), and pine biomass hydrolysate (PBH), were evaluated as potential carbon sources for R. eutropha 5119 and resulted in 1.8, 2.0, and 1.7 g/L PHA production, respectively. MBH proved the best carbon source, resulted in higher biomass (Y-x/s, 0.31 g/g) and PHA (Y-p/s, 0.14 g/g) yield.
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