Stability of aerobic granular sludge under condition of low influent C/N ratio: Correlation of sludge property and functional microorganism

Aerobic granular sludge process treating domestic wastewater with low C/N ratio is necessary to be studied for rapid urbanization in China and other countries. In this study, two parallel reactors with different influent C/N ratio (15 in R1, 5 in R2) were established. Compared to the disintegrated granule in R1 with high influent C/N ratio, granules with large size (650 mu m) and compact structure (integrity coefficient < 0.1) were stable in R2 along with influent C/N ratio decreased to 5. High-through sequencing illustrated the functional microbes like Thauera and Paracoccus enriched under low influent C/N ratio, and principal component analysis further showed these microbes were positive correlation with tryptophan and protein-like substances in extracellular polymeric substances (EPS) and granular strength. It was indicated that under low influent C/N ratio, several resistant microbes like Thauera (19.5%) enriched and then secreted tryptophan and protein-like substances, and stable granules with multi-functional microbes could be formed finally.