期刊
BIORESOURCE TECHNOLOGY
卷 270, 期 -, 页码 20-29出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.08.071
关键词
Dunaliella salina; Photobioreactor; Flocculation
资金
- UGC-UPE II
- Council of Scientific and Industrial Research, New Delhi, India [09/013/(0574)/2015-EMR-I, 09/013(0733)/2017-EMR-I]
- DST [R/DEV./D./DST-PURSE/5050/20244]
- UGC [22/12/2013(ii)EU-V]
Mass cultivation of Dunaliella salina was standardized in a flat plate photobioreactor followed by a vertical flat plate photobioreactor. Maximum biomass productivity (14.95 +/- 0.43 mgL(-1)d(-1) dry cell weight) was achieved in the latter at inoculum concentration of OD680nm = 0.1, 100 mu molm(-2)s(-1) light illumination and 1.0 L min(-1) aeration. Semicontinuous operation with varying KNO3 and NaHCO3 concentrations resulted highest biomass productivity (17.85 +/- 0.55 mgL(-1)d(-1)) at 0.50 mM NaHCO3 and 15 mM KNO3. However, maximum lipid (16.36 +/- 1.18% dry cell weight) was achieved at 0.75 mM NaHCO3 and 10 mM KNO3. Flocculation studies employing potash alum, FeCl3 center dot 6H(2)O or pH showed harvesting efficiencies exceeding 90% in 0.75 mM potash alum or FeCl3 center dot 6H(2)O or pH 11, but they yielded low concentration factor (< 5) and were detrimental (Fv/Fm < 0.50). A combination of 0.50mM FeCl3 center dot 6H(2)O and pH 9 was found as most suitable flocculating strategy with maximum concentration factor (> 14) and least damaging (Fv/Fm > 0.54).
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