期刊
BIORESOURCE TECHNOLOGY
卷 272, 期 -, 页码 26-33出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.09.069
关键词
Agave; Saccharification; Aspergillus niger; Pectinase; Low-cost enzyme cocktail
资金
- National Key Research and Development Projects Key Technological Study of Efficiently Cultivating the Ginkgo Tree and for Using the Leaf and Testa and Their Integrated Utilization [2017YFD0600701]
- National Natural Science Foundation of China [31730106]
- Jiangsu Postdoctoral Fund Mechanism of Efficient Accumulation of Flavonoids in Ginkgo Biloba Leaves Fermented by Aspergillus niger
- Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2017-05366]
To develop a cost-effective, time-saving and efficient saccharification system for converting biomass into mono-/oligo-saccharides for production of bioethanol or other biochemicals, a relatively low recalcitrant and widely available biomass Agave americana was selected as feedstock. During the investigation of efficient enzyme cocktail, pectinase, which usually is neglect for biomass saccharification, was confirmed that it dramatically improves the saccharification of agave biomass. A production-friendly fungal strain of Aspergillus niger Gyx086 was employed for low-cost enzyme cocktails production using wheat straw as substance. The enzyme cocktail which was with hyperactive pectinase activity of 6.29 +/- 0.42 U/ml could efficiently saccharify un-pretreated agave biomasses. As a result, under a mild condition at 35 degrees C in less than 72 h, most of the polysaccharides were completely converted into reducing sugar. The low-cost, process-simplified, and efficient biotechnology should stimulate the development of agave as feedstock for green energy and bio-based products production.
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