期刊
BIORESOURCE TECHNOLOGY
卷 155, 期 -, 页码 104-110出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.12.086
关键词
Poly(gamma-glutamic acid); Bacillus sutilis; Temperature; Metabolic flux; Enzyme activity
资金
- Director's Fund of Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering
- Innovation Project of Guangxi Graduate Education [YCBZ2012004]
- National Natural Science Foundation of China [21062001]
A thermophilic strain Bacillus subtilis GXA-28 with capability of gamma-PGA production was characterized, and its product was identified. The effect of temperatures on cell growth, gamma-PGA yield and molecular weight were investigated. Results showed that gamma-PGA yield reached 19.92 g/L at 45 degrees C with a high productivity of 0.91 g/L/h, and the molecular weight reached 3.03 x 10(6) Da. Then, the flux distribution and the key enzyme activities at 2-oxoglutarate branch under specified temperature were determined to illustrate the possible metabolic mechanism contributing to the improved gamma-PGA production. Results indicated that the fluxes from iso-citrate to 2-oxoglutarate and from 2-oxoglutarate to glutamate were increased with high activity of isocitrate dehydrogenase and glutamate dehydrogenase, which led to enhance gamma-PGA production. This work firstly employed temperature control strategy to improve gamma-PGA production, and provided novel information on the metabolic mechanism of gamma-PGA biosynthesis in Bacillus species. (C) 2013 Elsevier Ltd. All rights reserved.
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