期刊
BIORESOURCE TECHNOLOGY
卷 162, 期 -, 页码 331-340出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.03.152
关键词
Sequential digestion; Sludge pre-treatment; Sludge reduction; Methane production; Microbial community
资金
- Marine Biotechnology Program
- Ministry of Land, Transport and Maritime Affairs of the Korean Government
- Advanced Biomass R&D Center (ABC) of Korea
- Manpower Development Program for Marine Energy - Ministry of Ministry of Land, Transportation and Maritime Affairs (MLTM) of the Korean Government
- Ministry of Oceans and Fisheries, Korea
- POSCO
- Korea Institute of Energy Technology Evaluation and Planning (KETEP) - Korea Government Ministry of Knowledge Economy [2012A095]
- Ministry of Education, Science, and Technology [ABC-2012053889]
A lab-scale sequential sludge digestion process which consists of a mesophilic anaerobic digester (MAD) and a thermophilic aerobic digester (TAD) was developed. Thermal, thermal-alkaline and long-term alkaline pre-treatments were applied to the feed sludge to examine their effects on sludge removal and methane production. Especially after thermal-alkaline pre-treatment, high COD removal was maintained; methane production rate was also drastically increased by improving the hydrolysis step of sludge degradation. Polymerase chain reaction-denaturing gel gradient electrophoresis indicated that bacterial communities were represented by three phyla (Firmicutes, Proteobacteria, Actinobacteria) and that Clostridium straminisolvens was the major bacterial species in MAD. Quantitative real-time PCR results indicated that Methanosaeta concilli was the major archaeal species in MAD, and that Ureibacillus sp. was the most abundant bacterial species in TAD. (C) 2014 Elsevier Ltd. All rights reserved.
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