期刊
BIORESOURCE TECHNOLOGY
卷 169, 期 -, 页码 447-454出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.07.017
关键词
Miscanthus; Monolignins; Fermentation inhibitor; Mild alkali pretreatment; Biomass digestibility
资金
- 111 Project of Ministry of Education of China [B08032]
- Transgenic Plant and Animal Project of Ministry of Agriculture of China [2009ZX08009-119B]
- 973 Pre-project of Ministry of Science and Technology of China [2010CB134401]
- Open Projects of Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology [JSBEET1202]
In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions. Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold. In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p < 0.01. Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment. Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation. Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus. (C) 2014 Elsevier Ltd. All rights reserved.
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