期刊
BIORESOURCE TECHNOLOGY
卷 174, 期 -, 页码 81-87出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.09.147
关键词
Fumaric acid; Glycerol; Escherichia coli; Fed-batch culture; Acetate accumulation
资金
- National 973 Project [2011CBA00804, 2012CB725203]
- National Natural Science Foundation of China [NSFC-21176182, NSFC-21206112, NSFC-21390201]
- National High-tech R&D Program of China [2012AA02A702, 2012AA022103]
The evolved mutant Escherichia coli E2 previously developed for succinate production from glycerol was engineered in this study for fumaric acid production under aerobic conditions. Through deletion of three fumarases, 3.65 g/L fumaric acid was produced with the yield of 0.25 mol/mol glycerol and a large amount of acetate was accumulated as the main byproduct. In order to reduce acetate production several strategies were attempted, among which increasing the flux of the anaplerotic pathways through overexpression of phosphoenolpyruvate carboxylase gene ppc or the glyoxylate shunt operon aceBA effectively reduced acetate and improved fumaric acid production. In fed-batch culture, the resulting strain EF02(pSCppc) produced 41.5 g/L fumaric acid from glycerol with 70% of the maximum theoretical yield and an overall productivity of 0.51 g/L/h. (C) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据