期刊
BIORESOURCE TECHNOLOGY
卷 158, 期 -, 页码 355-359出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.02.071
关键词
Yeast; Carob pulp syrup; Sugarcane molasses; Carotenoids; Lipids; Flow cytometry
资金
- FCT (Fundacao para a Ciencia e a Tecnologia)
- FEDER funding through COMPETE - Programa Operacional Factores de Competitividade
The present work studied low-cost carbon sources for carotenoid and lipid production using the yeast Rhodosporidum toruloides NCYC 921. Carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in R. toruloides batch cultivations. Carob pulp syrup containing a total sugar concentration of 75 g L-1 induced the highest total fatty acid productivity (1.90 g L-1 h(-1)) and the highest carotenoid productivity (9.79 mu g L-1 h(-1)). Flow cytometric analysis revealed that most of the yeast cells (>60%) grown on carob pulp syrup displayed intact polarised membranes, conversely to the cells grown on sugarcane molasses, wherein a large proportion (>45%) displayed permeabilised cytoplasmic membranes. (C) 2014 Elsevier Ltd. All rights reserved.
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