期刊
BIORESOURCE TECHNOLOGY
卷 159, 期 -, 页码 167-175出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.02.021
关键词
Biorefineries; Rapeseed meal hydrolysate; Crude glycerol; 1,3-Propanediol; Clostridium butyricum
资金
- Andalusian Research, Innovation and Enterprise Council, Spain
- Spanish Ministry of Education and Science [ENE2010-15159]
Rapeseed meal (RSM) hydrolysate was evaluated as substitute for commercial nutrient supplements in 1,3-propanediol (PDO) fermentation using the strain Clostridium butyricum VPI 1718. RSM was enzymatically converted into a generic fermentation feedstock, enriched in amino acids, peptides and various micro-nutrients, using crude enzyme consortia produced via solid state fermentation by a fungal strain of Aspergillus oryzae. Initial free amino nitrogen concentration influenced PDO production in batch cultures. RSM hydrolysates were compared with commercial nutrient supplements regarding PDO production in fed-batch cultures carried out in a bench-scale bioreactor. The utilization of RSM hydrolysates in repeated batch cultivation resulted in a PDO concentration of 65.5 g/L with an overall productivity of 1.15 g/L/h that was almost 2 times higher than the productivity achieved when yeast extract was used as nutrient supplement. (C) 2014 Elsevier Ltd. All rights reserved.
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