期刊
BIORESOURCE TECHNOLOGY
卷 167, 期 -, 页码 433-440出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.06.044
关键词
(R, R)-2,3-butanediol; Vitamin C; Redox regulation; NADH/NAD(+) ratio; Paenibacillus polymyxa
资金
- National Basic Research Program of China (973 Program) [2014CB745100]
- National Natural Science Foundation of China [21176183]
- Natural Science Foundation of Tianjin [11JCZDJC16700]
- Program for New Century Excellent Talents in University'' of Ministry of Education [NCET-10-0609]
Cellular redox status and oxygen availability influence the product formation. Herein, decreasing agitation speed or adding vitamin C (Vc) achieved the 2,3-BDL yield of 0.40 g g(-1) or 0.39 g g(-1) glucose under batch fermentation, respectively. To our knowledge, this is the highest 2,3-BDL yield reported so far for Paenibacillus polymyxa without adding acetic acid. The NADH/NAD(+) ratio and 2,3-BDL titer could be increased significantly by reducing the agitation speed or adding Vc, indicating that the enhancement of 2,3-BDL is closely associated with the adjustment of NADH/NAD(+) ratio. Especially, Vc addition elevated the 2,3-BDL titer from 43.66 g L-1 to 71.71 g L-1 within 54 h under fed-batch fermentation. This is the highest titer of 2,3-BDL so far reported for P. polymyxa from glucose fermentation. This work provides a new strategy to improve 2,3-BDL production and helps us to understand the responses of P. polymyxa to extracellular oxidoreduction potential. (C) 2014 Elsevier Ltd. All rights reserved.
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