期刊
BIORESOURCE TECHNOLOGY
卷 166, 期 -, 页码 464-470出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.05.091
关键词
Aerobic granules; Storage; Signaling molecules; High throughput sequencing; Viable but non-culturable status
资金
- National Science Foundation of China [51278128]
- National Science Foundation for Young Scientists of China [41301332]
- State Key Laboratory of Pollution Control and Resource Reuse Foundation [PCRRF13009]
- Student Scientific Innovation Act of Fudan University
Long-term storage and successful reactivation after storage are essential for practical applications of aerobic granules on wastewater treatment. This study cultivated aerobic granules (SI) in sequencing batch reactors and then stored the granules at 4 degrees C in five liquid media (DI water (SW), acetone (SA), acetone/isoamyl acetate mix (SAA), saline water (SS), and formaldehyde (SF)) for over 1 year. The first four granules were then successfully reactivated in 24 h cultivation. The specific oxygen uptake rates (SOUR) of the granules followed SI > SS > SA > SAA > SW > SF; and the corresponding granular strengths (10 min ultrasound) followed SI > SA = SS > SAA > SW >> SF. During storage the granular cells secreted excess quantities of cyclic-diguanylate (c-di-GMP) and pentaphosphate (ppGpp) as responses to the stringent challenges. We proposed that to force cells in granules (Alphaproteobacteria, Flavobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Sphingobacteria, and Clostridia) entering viable but non-culturable (VBNC) status is the key of success for extended period storage of granules. (C) 2014 Elsevier Ltd. All rights reserved.
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