期刊
BIORESOURCE TECHNOLOGY
卷 152, 期 -, 页码 234-240出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.11.002
关键词
DHA; Schizochytrium sp.; k(L)a; OUR; Rheological property
资金
- Fundamental Research Funds of the Central Universities [JUSRP11226]
- National Key Technology R&D Program in the 12th Five Year Plan of China [2011BAD02B02]
Three independent cultures by fed batch strategy under different oxygen supply levels were investigated with Schizochytrium sp. S31 on glycerol in 50 L bioreactor. Three cultures all achieved high cell density cultivation (HCDC) with more than 100 g/L biomass density. However, the culture with middle oxygen supply level achieved the highest DHA concentration at 21.26 g/L. Dissolved oxygen (DO) limitation was commonly encountered in the present cultures, which was due to the dramatic decrease of k(L)a in high oxygen supply culture resulted from significantly increasing apparent viscosity of the broth. The rheological properties of the three cultures all exhibited shear-thinning behavior. The oxygen uptake rate (OUR) predominately influenced by k(L)a was suggested to replace DO as on-line control parameter for scale-up production of DHA. (C) 2013 Elsevier Ltd. All rights reserved.
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