期刊
BIORESOURCE TECHNOLOGY
卷 169, 期 -, 页码 723-730出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.07.039
关键词
Clostridium acetobutylicum; Immobilisation; LentiKats (R); Butanol; Fermentation
资金
- Research and Development Operational Programme - ERDF [ITMS 26240120016]
Clostridium acetobutylicum immobilised in polyvinylalcohol, lens-shaped hydrogel capsules (LentiKats (R)) was studied for production of butanol and other products of acetone-butanol-ethanol fermentation. After optimising the immobilisation protocol for anaerobic bacteria, continuous, repeated batch, and fed-batch fermentations in repeated batch mode were performed. Using glucose as a substrate, butanol productivity of 0.41 g/L/h and solvent productivity of 0.63 g/L/h were observed at a dilution rate of 0.05 h(-1) during continuous fermentation with a concentrated substrate (60 g/L). Through the process of repeated batch fermentation, the duration of fermentation was reduced from 27.8 h (free-cell fermentation) to 3.3 h (immobilised cells) with a solvent productivity of 0.77 g/L/h (butanol 0.57 g/L/h). The highest butanol and solvent productivities of 1.21 and 1.91 g/L/h were observed during fed-batch fermentation operated in repeated batch mode with yields of butanol (0.15 g/g) and solvents (0.24 g/g), respectively, produced per gram of glucose. (C) 2014 Elsevier Ltd. All rights reserved.
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