期刊
BIORESOURCE TECHNOLOGY
卷 169, 期 -, 页码 126-133出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.06.089
关键词
Urban wastewater treatment; Reverse-transcription qPCR; AOB; NOB; Multivariate analysis
资金
- Junta de Andalucia [NET 324936/1]
- Spanish Ministerio de Ciencia e Innovacion (MICINN) [CTM2010-17609/TECNO]
- Fondo Europeo de Desarrollo Regional (FEDER)
- MICINN
- Spanish Ministerio de Educacion
The abundance and transcription levels of specific gene markers of total bacteria, ammonia-oxidizing Betaproteobacteria, nitrite-oxidizing bacteria (Nitrospira-like) and denitrifiers (N2O-reducers) were analyzed using quantitative PCR (qPCR) and reverse-transcription qPCR during 9 months in a full-scale membrane bioreactor treating urban wastewater. A stable community of N-removal key players was developed; however, the abundance of active populations experienced sharper shifts, demonstrating their fast adaptation to changing conditions. Despite constituting a small percentage of the total bacterial community, the larger abundances of active populations of nitrifiers explained the high N-removal accomplished by the MBR. Multivariate analyses revealed that temperature, accumulation of volatile suspended solids in the sludge, BOD5, NH4+ concentration and C/N ratio of the wastewater contributed significantly (23-38%) to explain changes in the abundance of nitrifiers and denitrifiers. However, each targeted group showed different responses to shifts in these parameters, evidencing the complexity of the balance among them for successful biological N-removal. (C) 2014 Elsevier Ltd. All rights reserved.
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