期刊
BIORESOURCE TECHNOLOGY
卷 153, 期 -, 页码 47-54出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.11.059
关键词
Bioethanol; Microalgae; Chlorella vulgaris; Enzymatic saccharification; Nitrogen stress
资金
- Priority Research Centers Program through National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0020141]
- Forest Science AMP
- Technology Projects, Forest Service, Republic of Korea [S211313L010120]
- Korea Forest Service [S211313L010120] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The microalga Chlorella vulgaris is a potential feedstock for bioenergy due to its rapid growth, carbon dioxide fixation efficiency, and high accumulation of lipids and carbohydrates. In particular, the carbohydrates in microalgae make them a candidate for bioethanol feedstock. In this study, nutrient stress cultivation was employed to enhance the carbohydrate content of C. vulgaris. Nitrogen limitation increased the carbohydrate content to 22.4% from the normal content of 16.0% on dry weight basis. In addition, several pretreatment methods and enzymes were investigated to increase saccharification yields. Bead-beating pretreatment increased hydrolysis by 25% compared with the processes lacking pretreatment. In the enzymatic hydrolysis process, the pectinase enzyme group was superior for releasing fermentable sugars from carbohydrates in microalgae. In particular, pectinase from Aspergillus aculeatus displayed a 79% saccharification yield after 72 h at 50 degrees C. Using continuous immobilized yeast fermentation, microalgal hydrolysate was converted into ethanol at a yield of 89%. (C) 2013 Elsevier Ltd. All rights reserved.
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