期刊
BIORESOURCE TECHNOLOGY
卷 163, 期 -, 页码 48-53出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.04.021
关键词
Crude glycerol; Fumaric acid; Rhizopus arrhizus; Pellet; Co-fermentation
资金
- National Natural Science Foundation of China [21246005]
- Key Projects in the National Science & Technology Pillar Program during the 12th Five-year Plan Period [2011BAD22B04]
- Amoy Industrial Biotechnology R&D and Pilot Conversion Platform [3502Z20121009]
- Fundamental Research Funds for the Central University [ZY1331]
- Transformation of Agricultural Science and Technology Achievements Project [2013GB2C410539]
This work investigated the capability of Rhizopus arrhizus to assimilate biodiesel-derived crude glycerol and convert it into fumaric acid. After optimizing the initial glycerol concentration, spore inoculum and yeast extract concentration, smaller pellets (0.7 mm) and higher biomass (3.11 g/L) were obtained when R. arrhizus grew on crude glycerol. It was found that crude glycerol was more suitable than glucose for smaller R. arrhizus pellet forming. When 80 g/L crude glycerol was used as carbon source, the fumaric acid production of 4.37 g/L was obtained at 192 h. With a highest concentration of 22.81 g/L achieved in the co-fermentation of crude glycerol (40 g/L) and glucose (40 g/L) at 144 h, the fumaric acid production was enhanced by 553.6%, compared to the fermentation using glycerol (80 g/L) as sole carbon source. Moreover, the production cost of fumaric acid in co-fermentation was reduced by approximately 14% compared to glucose fermentation. (c) 2014 Elsevier Ltd. All rights reserved.
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