期刊
BIORESOURCE TECHNOLOGY
卷 170, 期 -, 页码 522-529出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.08.018
关键词
Astaxanthin; Stress; Metabolomics; Metabolic network; Haematococcus pluvialis
资金
- National High-tech RD Program (National 863'' program) [2012AA02A707]
- National Basic Research Program of China (National 973'' program) [2014CB745101, 2012CB721101, 2011CBA00803]
- Doctoral Program of Higher Education of China [20120032110020, 20130032120022]
Various combinations of acetate (Ac), Fe2+ and high light (HL) stress conditions were evaluated to maximize astaxanthin accumulation and biomass production in Haematococcus pluvialis, and then GC-MS and LC-MS based metabolomics were applied to determine molecular mechanisms responsible for enhancing astaxanthin accumulation under the stress conditions. With the optimized analytical protocols, the GC-MS and LC-MS analyses allowed identification of 93 stable and 24 unstable intracellular metabolites from H. pluvialis, respectively. In addition, a metabolic network was constructed based on GC-MS metabolomic datasets using a weighted correlation network analysis (WGCNA) approach. The network analysis uncovered 2, 1 and 1 distinguished metabolic modules highly associated with HL, Fe2+ & HL, and Ac & Fe2+ & HL conditions, respectively. Finally, LC-MS analysis found that AKG, Glu and R5P may be metabolites associated with the Fe2+ & HL condition. The study provided the first metabolomic view of cell growth and astaxanthin accumulation in H. pluvialis. (C) 2014 Elsevier Ltd. All rights reserved.
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