期刊
BIORESOURCE TECHNOLOGY
卷 135, 期 -, 页码 128-136出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.11.084
关键词
Nannochloropsis; Oils; Carotenoids; Biohydrogen; Biorefinely
资金
- Portuguese Foundation for Science and Technology (Fundacao para a Ciencia e a Tecnologia - FCT) [PTDC/AAC-AMB/100354/2008, SFRH/BPD/42004/2007]
- IPN [SIP 20110775]
- CONACyT
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/42004/2007] Funding Source: FCT
The microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO2 extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33 glipids/100 g(dry) biomass were found to be at 40 degrees C, 300 bar and a CO2 flow-rate of 0.62 g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO2 doped with 20% (w/w) ethanol was used, it was possible to extract 45 g(lipids)/100 g(dry) (biomass) of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6 mL/g(dry) (biomass). (C) 2012 Elsevier Ltd. All rights reserved.
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