期刊
BIORESOURCE TECHNOLOGY
卷 128, 期 -, 页码 156-163出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.10.098
关键词
Biocatalysis; Co-solvent; Hesperidinase; Ionic liquid; Isoquercitrin
资金
- Qing Lan Project of Jiangsu Province
- Science and Technology Support Program of Jiangsu Province [8E2010419]
- College Natural Science Research Project of Jiangsu Province [08KJB530002]
- Natural Science Foundation of China [21206061]
- Natural Science Foundation of Jiangsu Province [BK2009213]
- China Postdoctoral Science Foundation [2012M510124]
- Modern Agro-industry Technology Research System of China [CARS-22]
- Start Project for Introduced Talent at Jiangsu University of Science and Technology [35211002]
- Pre-research for an NSFC Project at Jiangsu University of Science and Technology [33201002]
An ionic liquid (IL)-containing buffer system was first applied in the conversion of rutin to isoquercitrin. High substrate solubility was achieved to enhance the selectivity and efficiency of hesperidinase-catalyzed reaction. Ten ILs were selected as co-solvents to assist catalytic reactions in this biotransformation process. The transformed products of rutin were identified by LC-MS. The [Bmim][BF4]-glycine-sodium hydroxide buffer (pH 9) (10:90, v/v) was found to be the best medium for the biotransformation of isoquercitrin from rutin with higher selectivity and efficiency. The reaction time was reduced by 0.33-fold while the conversion of rutin and the yield of isoquercitrin were increased by 1.67-fold and 233-fold. The results suggest that IL co-solvents have great potential to enhance the selectively enzymatic hydrolysis of rutin for isoquercitrin production. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据