期刊
BIORESOURCE TECHNOLOGY
卷 127, 期 -, 页码 337-342出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.09.116
关键词
Cupriavidus sp DT-1; Chlorpyrifos; 3,5,6-Trichloro-2-pyridinol; Degradation pathway
资金
- Chinese National Natural Science Foundation [31070099, 31000060, 30830001]
- Fundamental Research Funds for Environment and Plant Protection Institute, CATAS [010hzsZDZXZ001]
- Opening Fund of State Key Laboratory of Soil and Sustainable Agriculture [Y052010025]
A bacterial strain, Cupriavidus sp. DT-1, capable of degrading chlorpyrifos and 3,5,6-trichloro-2-pyridinol (TCP) and using these compounds as sole carbon source was isolated and characterized. Investigation of the degradation pathway showed that chlorpyrifos was first hydrolyzed to TCP, successively dechlorinated to 2-pyridinol, and then subjected to the cleavage of the pyridine ring and further degradation. The mpd gene, encoding the enzyme responsible for chlorpyrifos hydrolysis to TCP, was cloned and expressed in Escherichia coli BL21. Inoculation of chlorpyrifos-contaminated soil with strain DT-1 resulted in a degradation rate of chlorpyrifos and TCP of 100% and 94.3%, respectively as compared to a rate of 28.2% and 19.9% in uninoculated soil. This finding suggests that strain DT-1 has potential for use in bioremediation of chlorpyrifos-contaminated environments. (C) 2012 Elsevier Ltd. All rights reserved.
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