期刊
BIORESOURCE TECHNOLOGY
卷 127, 期 -, 页码 291-297出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.09.111
关键词
Cellulosic ethanol; Fed-batch enzymatic saccharification and fermentation; Pretreatment; Woody biomass; Biofuel and biorefinery
资金
- USDA Small Business Innovative Research (SBIR) Phase II project [2010-33610-21589]
- NIFA [2010-33610-21589, 580375] Funding Source: Federal RePORTER
Lodgepole wood chips were pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) at 25% solids loading and 180 degrees C for 20 min with sulfuric acid and sodium bisulfite charges of 2.2 and 8 wt/wt% on an oven-dry wood basis, respectively. The pretreated wood chips were disk-milled with pretreatment spent liquor and water, and the solid fraction was separated from the liquor stream. The liquor was neutralized and concentrated through vacuum evaporation. Quasi-simultaneous enzymatic saccharification of the cellulosic solids and combined fermentation with the concentrated liquor was conducted at up to 20% total solids loading. Fed-batching of the solids facilitated liquefaction and saccharification, as well as managing instantaneous inhibitor concentrations. At a commercial cellulase (CTec2) loading of only 9 FPU or 0.06 mL/g untreated wood, a maximum ethanol titer of 47.4 g/L was achieved, resulting in a calculated yield of 285 L/tonne of wood using Saccharomyces cerevisiae YRH400 at 35 degrees C and pH 5.5. Published by Elsevier Ltd.
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