期刊
BIORESOURCE TECHNOLOGY
卷 131, 期 -, 页码 121-127出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.12.134
关键词
Biogas; Methanogen; Protein; mcrA gene; 16S rRNA gene
资金
- EU [HUSRB/1002/214/041 IPA, HURO/1001/193/2.2.2 CBC, IEE/10/235 SI2.591589 Green-GasGrids]
- Domestic funds [TAMOP-4.2.1/B-09/1/KONV-2010-0005, TAMOP-4.2.2/B-10/1-2010-0012]
Terminal restriction fragment length polymorphism (T-RFLP) was applied to study the changes in the composition of the methanogens of biogas-producing microbial communities on adaptation to protein-rich monosubstrates such as casein and blood. Specially developed laboratory scale (5-L) continuously stirred tank reactors have been developed and used in these experiments. Sequencing of the appropriate T-RF fragments selected from a methanogen-specific (mcrA gene-based) library revealed that the methanogens responded to the unconventional substrates by changing the community structure. T-RFLP of the 16S rDNA gene confirmed the findings. (C) 2012 Elsevier Ltd. All rights reserved.
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