期刊
BIORESOURCE TECHNOLOGY
卷 149, 期 -, 页码 169-176出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.09.047
关键词
L-Lactic acid; Bacillus sp.; 2-Furfural; Cellulosic hydrolysate
资金
- Chinese National Programs for High Technology Research and Development [2011AA02A202]
- Knowledge Innovation Program of the Chinese Academy of Sciences [KSZD-EW-Z-016-3]
- National Natural Science Foundation of China [31270108]
In this study, efficient polymer-grade L-lactic acid production was achieved with the strain Bacillus sp. P38 by using cellulosic hydrolysate as the sole carbon source. In fed-batch fermentation, 180 g L-1 L-lactic acid was obtained with a volumetric productivity of 2.4 g L-1 h(-1) and a yield of 0.96 g g(-1) total reducing sugars. No D-isomer of lactic acid was detected in the broth. Strain P38 tolerated up to 10 g L-1 2-furfural, and lactate production was sharply inhibited only when the 2-furfural concentration was higher than 6 g L-1. Moreover, strain P38 also tolerated high concentrations (>6 g L-1) of other fermentation inhibitors in cellulosic hydrolysate, such as vanillin and acetic acid, although it was slightly sensitive to formic acid. The efficient L-lactic acid production, combined with high inhibitor tolerance and efficient pentose utilization, indicate that Bacillus sp. P38 is a promising producer of polymer-grade L-lactic acid from cellulosic biomass. (C) 2013 Elsevier Ltd. All rights reserved.
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