期刊
BIORESOURCE TECHNOLOGY
卷 135, 期 -, 页码 2-6出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.01.047
关键词
Iron oxide; Cellulase; Lignocellulose; Biofuel; Nanobiotechnology
The objective of the present work was to develop a thermostable beta-glucosidase through immobilization on a nanoscale carrier for potential application in biofuel production. beta-Glucosidase (BGL) from Aspergillus niger was immobilized to functionalized magnetic nanoparticles by covalent binding. Immobilized nanoparticles showed 93% immobilization binding. Immobilized and free BGL were characterized using Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) techniques. Free and immobilized enzyme exhibited different pH-optima at pH 4.0 and 6.0, respectively, but had the same temperature optima at 60 degrees C. Michaelis constant (K-M) was 3.5 and 4.3 mM for free and immobilized BGL. Thermal stability of the immobilized enzyme was enhanced at 70 degrees C. The immobilized nanoparticleenzyme conjugate retained more than 50% enzyme activity up to the 16th cycle. Maximum glucose synthesis from cellobiose hydrolysis by immobilized BGL was achieved at 16 h. (C) 2013 Elsevier Ltd. All rights reserved.
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