期刊
BIORESOURCE TECHNOLOGY
卷 145, 期 -, 页码 182-187出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.01.046
关键词
Biobutanol; Rice straw hydrolysate; Clostridium sporogenes; ABE fermentation; Detoxification
资金
- Council of Scientific and Industrial Research
Biobutanol from lignocellulosic biomass has gained much attention due to several advantages over bioethanol. Though microbial production of butanol through ABE fermentation is an established technology, the use of lignocellulosic biomass as feedstock presents several challenges. In the present study, biobutanol production from enzymatic hydrolysate of acid pretreated rice straw was evaluated using Clostridium sporogenes BE01. This strain gave a butanol yield of 3.43 g/l and a total solvent yield of 5.32 g/l in rice straw hydrolysate supplemented with calcium carbonate and yeast extract. Hydrolysate was analyzed for the level of inhibitors such as acetic acid, formic acid and furfurals which affect the growth of the organism and in turn ABE fermentation. Methods for preconditioning the hydrolysate to remove toxic end products were done so as to improve the fermentation efficiency. Conditions of ABE fermentation were fine tuned resulting in an enhanced biobutanol reaching 5.52 g/l. (C) 2013 Elsevier Ltd. All rights reserved.
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