4.8 Article

Characterization of a renewable extracellular polysaccharide from defatted microalgae Dunaliella tertiolecta

期刊

BIORESOURCE TECHNOLOGY
卷 129, 期 -, 页码 343-350

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.11.077

关键词

Microalgae; Polysaccharide; Monosaccharide; FT-IR; NMR; Biorefinery

资金

  1. Ministry of Land, Transport and Maritime Affairs of Korean Government
  2. Ministry of Education of the Czech Republic [CEZ: MSM6046137305]
  3. Korea Institute of Marine Science & Technology Promotion (KIMST) [200902672] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
  4. Rural Development Administration (RDA), Republic of Korea [PJ009149012013] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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Extracellular polysaccharide (EPS) was isolated from defatted micro-algae Dunaliela tertiolecta and defined as linear (1 -> 4)-alpha-D-glucan based on monosaccharide composition, enzymatic and spectroscopic analyses. Optimization and characterization of acidic and enzymatic hydrolyses of EPS have been performed for its potential use as a renewable biorefinery material. The hydrolytic methods were improved to assess the effect of substrate specificity, reaction time, pH, ionic strength and temperature on efficiency of glucose production. EPS was effectively converted into glucose within one-step enzymatic or acidic hydrolysis under optimized conditions. Over 90% recovery of glucose was achieved for both hydrolytic approaches. High potential production of EPS and high yield conversion of this substrate to glucose may allow further exploration of microalga D. tertiolecta as a potential biomass producer for biotechnological and industrial exploitation of bioethanol. (C) 2012 Elsevier Ltd. All rights reserved.

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