期刊
BIORESOURCE TECHNOLOGY
卷 136, 期 -, 页码 496-501出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.03.072
关键词
Microalgae; CO2 fixation; Nuclear irradiation; Domestication; Biomass
资金
- National Natural Science Foundation of China [51176163]
- International Sci. & Tech. Cooperation Program of China [2012DFG61770, 2010DFA72730]
- National High Technology R&D Program of China [2012AA050101]
- National Key Technology R&D Program of China [2011BAD14B02]
- Specialized Research Fund for the Doctoral Program of Higher Education [20110101110021]
- Program for New Century Excellent Talents in University [NCET-11-0446]
- Key Natural Science Foundation of Zhejiang Province [Z1090532]
- Program of Introducing Talents of Discipline to University [B08026]
To improve biomass productivity and CO2 fixation of microalgae under 15% (v/v) CO2 of flue gas, Chlorella species were mutated by nuclear irradiation and domesticated with high concentrations of CO2. The biomass yield of Chlorella pyrenoidosa mutated using 500 Gy of Co-60 gamma irradiation increased by 53.1% (to 1.12 g L-1) under air bubbling. The mutants were domesticated with gradually increased high concentrations of CO2 [from 0.038% (v/v) to 15% (v/v)], which increased the biomass yield to 2.41 g L-1. When light transmission and culture mixing in photo-bioreactors were enhanced at 15% (v/v) CO2, the peak growth rate of the domesticated mutant (named Chlorella PY-ZU1) was increased to 0.68 g L-1 d(-1). When the ratio of gas flow rate (L min(-1)) to 1 L of microalgae culture was 0.011, the peak CO2 fixation rate and the efficiency of Chlorella PY-ZU1 were 1.54 g L-1 d(-1) and 32.7%, respectively. (C) 2013 Elsevier Ltd. All rights reserved.
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