期刊
BIORESOURCE TECHNOLOGY
卷 146, 期 -, 页码 274-281出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.07.081
关键词
2,3-Butanediol (2,3-BD); Saccharomyces cerevisiae; Pyruvate decarboxylase-deficient mutant; MTH1
资金
- Advanced Biomass R&D Center (ABC) of Korea Grant [2010-0029799]
- Ministry of Science, ICT & Future Planning
- Korea Research Council of Fundamental Science & Technology (KRCF) Grant
In order to produce 2,3-butanediol (2,3-BD) with a high titer, it is necessary to engineer Saccharomyces cerevisiae by deleting the competing pathway and overexpressing the 2,3-BD biosynthetic pathway. A pyruvate decarboxylase (Pdc)-deficient mutant was constructed and evolved for rapid glucose consumption without ethanol production. Genome re-sequencing of the evolved strain (SOS4) revealed a point mutation (A81P) in MTH1 coding for a transcriptional regulator involved in glucose sensing, unlike the previously reported Pdc-deficient mutant which had internal deletion in MTH1. When alsS and alsD genes from Bacillus subtilis, and endogenous BDH1 gene were overexpressed in SOS4, the resulting strain (BD4) not only produced 2,3-BD efficiently, but also consumed glucose faster than the parental strain. In fed-batch fermentation with optimum aeration, 2,3-BD concentration increased up to 96.2 g/L. These results suggest that S. cerevisiae might be a promising host for producing 2,3-BD for industrial applications. (c) 2013 Elsevier Ltd. All rights reserved.
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