期刊
BIORESOURCE TECHNOLOGY
卷 148, 期 -, 页码 394-400出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.08.114
关键词
Adaptive evolution; Escherichia coli; L(+)-Lactic acid; Molasses; Sucrose fermentation
资金
- Natural Science Foundation of Hubei Province [2011CDB076]
- Hubei University of Technology
- Chutian Scholar Program of Hubei Province, P.R. China
- Northern Illinois University, USA
The D-lactic acid producing strain, Escherichia coli HBUT-D, was reengineered for L(+)-lactic acid fermentation by replacing the D-lactate dehydrogenase gene (ldhA) with an L(+)-lactate dehydrogenase gene (ldhL) from Pedicoccus acidilactici, followed by adaptive evolution in sucrose. The resulting strain, WYZ-L, has enhanced expression of the sucrose operon (cscA and cscKB). In 100 g L-1 of sucrose fermentation using mineral salt medium, WYZ-L produced 97 g L-1 of L(+)-lactic acid, with a yield of 90%, a maximum productivity of 3.17 g L-1 h(-1) and an optical purity of greater than 99%. In fermentations using sugarcane molasses and corn steep liquor without additional nutrients, WYZ-L produced 75 g L-1 of L(+)-lactic acid, with a yield of 85%, a maximum productivity of 1.18 g L-1 h(-1), and greater than 99% optical purity. These results demonstrated that WYZ-L has the potential to use waste molasses and corn steep liquor as a resource for L(+)-lactic acid fermentation. (C) 2013 Elsevier Ltd. All rights reserved.
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