期刊
BIORESOURCE TECHNOLOGY
卷 148, 期 -, 页码 202-207出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.08.143
关键词
Burkholderia cepacia; Modified attapulgite; Biodiesel production; Immobilized lipase
资金
- Open Project of Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology [JSBEET1203]
- Colleges and Universities in Jiangsu Province Natural Science Fund [12KJB480003]
- Open Project of Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration [JPELBCPL2012003]
- Jiangsu Province Ordinary University Innovative Research Program [CXLX11_0602, CXZZ12_0699]
Lipase from Burkholderia cepacia was immobilized on modified attapulgite by cross-linking reaction for biodiesel production with jatropha oil as feedstock. Effects of various factors on biodiesel production were studied by single-factor experiment. Results indicated that the best conditions for biodiesel preparation were: 10 g jatropha oil, 2.4 g methanol (molar ratio of oil to methanol is 1:6.6) being added at 3 h intervals, 7 wt% water, 10 wt% immobilized lipase, temperature 35 degrees C, and time 24 h. Under these conditions, the maximum biodiesel yield reached 94%. The immobilized lipase retained 95% of its relative activity during the ten repeated batch reactions. The half-life time of the immobilized lipase is 731 h. Kinetics was studied and the V,a of the immobilized lipases were 6.823 mmol L-1. This immobilized lipase catalyzed process has potential industrial use for biodiesel production to replace chemical-catalyzed method. (C) 2013 Elsevier Ltd. All rights reserved.
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