期刊
BIORESOURCE TECHNOLOGY
卷 128, 期 -, 页码 526-532出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.10.107
关键词
Biodegradation; Di-n-butyl phthalate; Consortium; DGGE; GC/MS
资金
- National Natural Science Foundation of China [31170115]
- Major Science and Technology Program for Water Pollution Control and Treatment [2012ZX07101-012]
HD-1, a stable microbial consortium capable of mineralizing di-n-butyl phthalate (DBP), was developed from activated sludge. The dominant microorganisms in the consortium, Gordonia sp., Burkholderia sp. and Achromobacter sp., were identified by denaturing gradient gel electrophoresis (DGGE). The consortium could mineralize approximately 90% of 1200 mg/L DBP after 48 h of cultivation. The optimal DBP degradation conditions were 25-30 degrees C and pH 8.0-9.0. The addition of yeast (0.5 g/L), sodium acetate (0.5 g/L, 1.0 g/L), Brij 35 (0.2%, 0.5%, 1.0%), or Triton X-100 (0.2%) enhanced DBP degradation. The DBP degradation rate was influenced by the presence of dimethyl phthalate (DMP) and diethyl phthalate (DEP). Only one main intermediate, phthalic acid, could be monitored by gas chromatography-mass spectrometry (GC-MS) during the degradation process. The HD-1 consortium also utilized phenol, o-dihydroxybenzene as the sole carbon and energy source. The results indicate the consortium may represent a promising application for DBP bioremediation. (C) 2012 Elsevier Ltd. All rights reserved.
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