期刊
BIORESOURCE TECHNOLOGY
卷 128, 期 -, 页码 703-710出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.10.106
关键词
Hydrogen-oxidizing autotrophic denitrification; Enrichment; High-throughput sequencing; Thauera
资金
- Hong Kong General Research Fund [7190/12E]
- University of Hong Kong
The present study, for the first time, reported a Thauera-dominated hydrogen-oxidizing autotrophic denitrifying microbial community enriched from different seed sludges including activated sludge and anaerobic digestion sludge. After 244 days enrichment, nitrogen removal rates reached up to 0.2 mg N/mg VSS/d which were comparable to that of the model organism Paracoccus denitrificans under the same conditions. Furthermore, high-throughput sequencing was applied to characterize and compare the seed sludges and enriched cultures. Operational taxonomic units (OTU)-based analysis (97% similarity cutoff) of total 280,000 16S rRNA gene V6 region sequences from 7 sludge samples (40,000 sequences per sample) revealed that the microbial diversity decreased after the enrichment, indicated by OTh numbers drop of 55-60%. Thauera species in the class of p-Proteobacteria were enriched into the dominant populations with relative abundances of 47-62%, regardless of seed sludge sources. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据