期刊
BIORESOURCE TECHNOLOGY
卷 133, 期 -, 页码 500-506出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.01.134
关键词
Bio-oil; Chlamydomonas reinhardtii; Activated carbon; Metabolic evolution; Lipid production
资金
- National Science Foundation [EPS-1101284, CBET-1133319]
- Iowa Energy Center [12-06]
- Iowa State University Bailey Award
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1133319] Funding Source: National Science Foundation
- EPSCoR
- Office Of The Director [1101284] Funding Source: National Science Foundation
This work was to utilize acetic acid contained in bio-oil for growth and lipid production of the microalga Chlamydomonas reinhardtii. The acetic acid-rich bio-oil fraction derived from fast pyrolysis of softwood contained 26% (w/w) acetic acid, formic acid, methanol, furfural, acetol, and phenolics as identified compounds, and 13% (w/w) unidentified compounds. Among those identified compounds, phenolics were most inhibitory to algal growth, followed by furfural and acetol. To enhance the fermentability of the bio-oil fraction, activated carbon was used to reduce the toxicity of the bio-oil, while metabolic evolution was used to enhance the toxicity tolerance of the microalgae. Combining activated carbon treatment and using evolved algal strain resulted in significant algal growth improvement. The results collectively showed that fast pyrolysis-fermentation process was a viable approach for converting biomass into fuels and chemicals. (C) 2013 Elsevier Ltd. All rights reserved.
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