期刊
BIORESOURCE TECHNOLOGY
卷 135, 期 -, 页码 410-416出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.06.092
关键词
Biodiesel fuel; Biomass support particles; Esterification; Immobilized cells; Lipase
资金
- Regional Innovation Creation RD Programs
- Ministry of Economy, Trade and Industry (METI)
- Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan
For enzymatic biodiesel production from plant oil hydrolysates, an Aspergillus oryzae whole-cell biocatalyst that expresses Candida antarctica lipase B (r-CALB) with high esterification activity was developed. Each of soybean and palm oils was hydrolyzed using Candida rugosa lipase, and the resultant hydrolysates were subjected to esterification where immobilized r-CALB was used as a catalyst. In esterification, r-CALB afforded a methyl ester content of more than 90% after 6 h with the addition of 1.5 M equivalents of methanol. Favorably, stepwise additions of methanol and a little water were unnecessary for maintaining the lipase stability of r-CALB during esterification. During long-term esterification in a rotator, r-CALB can be recycled for 20 cycles without a significant loss of lipase activity, resulting in a methyl ester content of more than 90% even after the 20th batch. Therefore, the presented reaction system using r-CALB shows promise for biodiesel production from plant oil hydrolysates. (C) 2012 Elsevier Ltd. All rights reserved.
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